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Experimental study demonstrates reliable colony growth in unicellular blue-green algae, highlighting the necessity of low agar concentration and separate sterilization.
The efficiencies of plating of 2 cultures of unicellular blue-green algae, 1 coccoid and 1 rod-shaped, were studied systematically. Reproducible colony growth and accurate viable counts are dependent on the use of a low agar concentration, and on the sterilization of the agar separately from the mineral components of the medium.
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Mary M. Allen (1968) studied this question.
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