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Develop and validate a rapid, reliable technique for HLA class I and class II typing using immunomagnetically isolated cells directly from blood.

November 1, 1986Tissue Antigens

HLA class I and II typing using cells positively selected from blood by immunomagnetic isolation ‐ a fast and reliable technique

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Develop and validate a rapid, reliable technique for HLA class I and class II typing using immunomagnetically isolated cells directly from blood.
Magnetic microspheres coated with monoclonal antibodies against CD8 or HLA class II monomorphic epitopes were used to isolate target cells from ACD blood within 15 minutes.
Isolated bead-bound cells underwent microcytotoxic HLA typing with a 55-minute incubation, using acridine orange and ethidium bromide staining to differentiate viable and non-viable cells.
Assay concordance was evaluated against conventional typing across 50 healthy donors and 10 patients for HLA-ABC, and 25 healthy donors and 30 patients for HLA-DR.
The entire immunomagnetic typing procedure required only 70 minutes and demonstrated higher sensitivity and lower background staining than conventional microcytotoxicity assays.
Concordance rates with conventional HLA typing ranged from 92% to 100% across healthy and patient cohorts.
The technique achieved successful HLA class II typing in six clinical samples where conventional typing failed, while also proving effective for cadaveric donor blood.

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Implication

Diagnostic study demonstrates rapid HLA class I and II typing using immunomagnetic isolation in human blood, highlighting superior sensitivity over conventional microcytotoxicity assays.

Cite This Study

Vartdal et al. (1986) studied this question.

synapsesocial.com/papers/6a1d9dbb1c2cbcb15c5e9104 https://doi.org/10.1111/j.1399-0039.1986.tb00500.x

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Implication

Diagnostic study demonstrates rapid HLA class I and II typing using immunomagnetic isolation in human blood, highlighting superior sensitivity over conventional microcytotoxicity assays.

HLA class I and II typing using cells positively selected from blood by immunomagnetic isolation ‐ a fast and reliable technique

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Discussion

Implication

Cite This Study

HLA class I and II typing using cells positively selected from blood by immunomagnetic isolation ‐ a fast and reliable technique

Key Points

Discussion

Implication

Cite This Study

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