Characterization of glomerular extracellular matrix in IgA nephropathy by proteomic analysis of laser-captured microdissected glomeruli

Figures

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  Fig. 1. a We combined our protein list of quantified proteins with the protein list from the Matrisome project database (n = 1027) and the list from Lennon et al. (n = 144 proteins). b. Comparison of our list of ECM proteins with the protein list from Lennon et al. [7] and Hobeika et al. [8]

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  Fig. 2. Scatter plot illustrating label-free quantification intensities for Periostin between groups. Mean (standard deviation) and p-values are given

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  Fig. 3. a-d Periostin staining in the three patient groups a) Control patient with negative staining b) Non-progressive IgAN patient and c) Progressive IgAN patient with more positive staining. There is week periostin staining for both IgAN without and with progression. d) Example of Aperio pixel analysis of glomeruli in 3 c), orange and yellow indicate positive pixels and blue indicating negative pixels

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  Fig. 4. a Principal component analysis plot of extracellular matrix proteins. Green represents control patients; blue represents IgAN without progression and red IgAN patients with progression. b Unsupervised hierarchical clustering map of the 20 most differently abundant glomerular proteins in patients with IgAN as compared to controls. Each vertical bar represents a patient/control and each horizontal bar represents a protein. Red indicates more abundant proteins while green less abundant proteins in IgAN vs controls. IgA + indicate progressive IgAN patients, IgA – indicate non progressors

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  Fig. 5. Glomerular ECM protein interaction network. Proteins significantly changed in IgAN glomeruli as compared to control glomeruli were included in the analysis using the STRING database with medium confidence score (0.4). Lines of different color represent types of evidence used in predicting associations. Red line: fusion evidence; green line: neighborhood evidence; blue line: co-occurrence evidence; purple line: experimental evidence; yellow line: text mining evidence; light blue line: database evidence; black line: co-expression evidence. Figure is exported from the string website