What question did this study set out to answer?

To compare the discriminatory power, reproducibility, typability, and practical utility of cross-streaking and mitomycin C-induced spotting methods for bacteriocin typing of Serratia marcescens.

January 1, 1983Journal of Clinical MicrobiologyOpen Access

Comparison of two methods for bacteriocin typing of Serratia marcescens

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Authors

PLP. LaiNational Pingtung University of Science and Technology YNYun Fong NgeowUniversitas Teknologi Sulawesi SPS D PuthuchearyUniversity of Malaya

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Implication

Comparative laboratory study demonstrates superior discrimination and typability with cross-streaking over spotting in Serratia marcescens isolates, indicating its utility for epidemiological tracing.

Key Points

To compare the discriminatory power, reproducibility, typability, and practical utility of cross-streaking and mitomycin C-induced spotting methods for bacteriocin typing of Serratia marcescens.
Evaluated 80 epidemiologically unrelated Serratia marcescens isolates collected from patients in a single hospital.
Typed all isolates using both the cross-streaking method and the mitomycin C-induced spotting method.
Assessed both typing methods for discrimination capacity, reproducibility, percentage typability, cost, and technical complexity.
The cross-streaking method differentiated 50 bacteriocin groups out of the 80 isolates compared to 31 groups differentiated by the spotting method.
The cross-streaking method achieved higher reproducibility (82.5% vs. 78.75%) and typability (93.75% vs. 90.0%) than the spotting method.
Cross-streaking provided lower operating costs, technical simplicity, and greater ease in scoring results compared to mitomycin C induction.

Cite This Study

Lai et al. (1983) studied this question.

synapsesocial.com/papers/6a1da2fe750575be8d2faed1 https://doi.org/10.1128/jcm.17.1.1-6.1983

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Comparison of two methods for bacteriocin typing of Serratia marcescens

Discussion

Member takes

Implication

Key Points

Cite This Study

Comparison of two methods for bacteriocin typing of Serratia marcescens

Discussion

Member takes

Implication

Key Points

Cite This Study

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