What question did this study set out to answer?

To isolate, clone, and characterize the murine stearoyl-CoA desaturase 2 (SCD2) gene and assess its expression during preadipocyte differentiation and across various tissues and dietary states.

September 1, 1989Journal of Biological ChemistryOpen Access

Differentiation-induced Gene Expression in 3T3-L1 Preadipocytes

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To isolate, clone, and characterize the murine stearoyl-CoA desaturase 2 (SCD2) gene and assess its expression during preadipocyte differentiation and across various tissues and dietary states.
Isolated and sequenced mouse SCD2 cDNA and its 15-kilobase genomic clone from 3T3-L1 cells, identifying transcription start sites, exons, and promoter motifs.
Quantified SCD2 mRNA expression across 3T3-L1 preadipocyte differentiation and in mouse tissues under fat-containing versus fat-free diets using RNase protection and primer extension assays.
SCD2 cDNA encodes a 358-amino acid protein sharing >87% identity with SCD1, exhibiting a 10-fold increase in mRNA abundance during 3T3-L1 preadipocyte differentiation.
SCD2 is expressed constitutively in brain but absent in liver, with expression in kidney, adipose, and lung tissues strongly induced when mice are switched from an unsaturated fat diet to a fat-free diet.
The SCD2 gene spans six exons and five introns, lacking a canonical TATA box but containing two CCAAT boxes and promoter regions homologous to Sp1 and glucocorticoid regulatory motifs.

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Implication

Laboratory study reveals differentiation- and diet-regulated expression of stearoyl-CoA desaturase 2 in mice, indicating distinct tissue-specific control of fat synthesis enzymes.

Cite This Study

Kaestner et al. (1989) studied this question.

synapsesocial.com/papers/6a1dbff6c238e2f45834cc57 https://doi.org/10.1016/s0021-9258(18)63763-9

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Implication

Laboratory study reveals differentiation- and diet-regulated expression of stearoyl-CoA desaturase 2 in mice, indicating distinct tissue-specific control of fat synthesis enzymes.

Differentiation-induced Gene Expression in 3T3-L1 Preadipocytes

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Discussion

Implication

Cite This Study

Differentiation-induced Gene Expression in 3T3-L1 Preadipocytes

Key Points

Discussion

Implication

Cite This Study

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