What question did this study set out to answer?

To establish an in vitro culture technique for growing human bone marrow cell colonies in an agar-gel medium.

August 1, 1970Journal of Cellular Physiology

Human bone marrow colony growth in agar‐gel

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Authors

BPBeverley L. PikeThe Royal Melbourne Hospital WRWilliam A. RobinsonUniversity of Colorado Anschutz Medical Campus

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Implication

In vitro study demonstrates granulocytic colony formation from human bone marrow using leukocyte feeder layers, highlighting a viable method for analyzing human hematopoiesis.

Key Points

To establish an in vitro culture technique for growing human bone marrow cell colonies in an agar-gel medium.
Bone marrow aspirates collected in heparinized syringes were plated at 2 × 10^5 cells into agar-gel medium.
Feeder layers containing 1 × 10^6 normal human peripheral white blood cells were utilized as the biological growth stimulus.
Normal human bone marrow yielded 32–102 colonies per 2 × 10^5 plated cells, with colonies consisting almost exclusively of granulocytes.
Colonies grew at a slower rate than murine bone marrow but reached a comparable size of 500–1,500 cells by days 12–16.

Cite This Study

Pike et al. (1970) studied this question.

synapsesocial.com/papers/6a1e6c6c09554abc3868cb19 https://doi.org/10.1002/jcp.1040760111

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Implication

In vitro study demonstrates granulocytic colony formation from human bone marrow using leukocyte feeder layers, highlighting a viable method for analyzing human hematopoiesis.

Human bone marrow colony growth in agar‐gel

Discussion

Member takes

Implication

Key Points

Cite This Study

Human bone marrow colony growth in agar‐gel

Discussion

Member takes

Implication

Key Points

Cite This Study

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