A simple, rapid, precise, and economical analytical method was developed for the simultaneous estimation of Glimepiride and Atorvastatin Calcium by RP-HPLC and UV spectroscopy in bulk and tablet dosage forms. Chromatographic separation in RP-HPLC was achieved using a C18 column with an optimized mobile phase under isocratic conditions. Both drugs showed well-resolved peaks with satisfactory retention times and system suitability parameters. The UV spectroscopic method was developed using simultaneous equation and absorbance correction methods at selected wavelengths. The developed methods were validated according to ICH guidelines. Validation parameters included linearity, accuracy, precision, specificity, robustness, LOD, and LOQ. Excellent linearity was observed with correlation coefficients greater than 0.999 for both drugs. Recovery studies confirmed the accuracy of the methods, while low %RSD values indicated good precision and reproducibility. The RP-HPLC method showed higher sensitivity and specificity compared to UV spectroscopy. The proposed methods were found suitable for routine quality control analysis of combined pharmaceutical formulations containing Glimepiride and Atorvastatin Calcium.
1*Mahajan Vivek R., 2Jalde Swapna R., 3Dr. Ingole R. D. (Mon,) studied this question.