What question did this study set out to answer?

To identify the predominant species and clonal diversity of Gram-positive pleomorphic rods, specifically Actinomyces, isolated from infected dentin in active root caries lesions.

September 1, 1999Journal of Dental Research

The Predominant Actinomyces spp. Isolated from Infected Dentin of Active Root Caries Lesions

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To identify the predominant species and clonal diversity of Gram-positive pleomorphic rods, specifically Actinomyces, isolated from infected dentin in active root caries lesions.
Sampled infected dentin from active root caries lesions (n = 9) and cultured 654 Gram-positive pleomorphic rod isolates under aerobic and anaerobic conditions.
Identified isolates to species level via biochemical and physiological tests, and evaluated clonal diversity of Actinomyces israelii (n = 56) and Actinomyces gerencseriae (n = 46) using repetitive extragenic palindromic PCR (REP-PCR).
Among 654 isolates, 607 belonged to Actinomyces, dominated by Actinomyces israelii (n = 242) and Actinomyces gerencseriae (n = 225), whereas Actinomyces naeslundii accounted for 109 isolates.
Isolation rates differed significantly by atmospheric condition, with Actinomyces gerencseriae predominantly isolated from aerobic plates and Actinomyces israelii from anaerobic plates (p < 0.05 for both).
REP-PCR demonstrated heterogeneous genotypes within individual lesions, with distinct DNA patterns between Actinomyces gerencseriae and Actinomyces israelii strains.

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Implication

Microbiological study identifies Actinomyces israelii and Actinomyces gerencseriae as predominant species in active root caries lesions, highlighting diverse bacterial colonization.

Cite This Study

Brailsford et al. (1999) studied this question.

synapsesocial.com/papers/6a1e7a068a8a69122355ec4a https://doi.org/10.1177/00220345990780090701

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Implication

Microbiological study identifies Actinomyces israelii and Actinomyces gerencseriae as predominant species in active root caries lesions, highlighting diverse bacterial colonization.

The Predominant Actinomyces spp. Isolated from Infected Dentin of Active Root Caries Lesions

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Discussion

Implication

Cite This Study

The Predominant Actinomyces spp. Isolated from Infected Dentin of Active Root Caries Lesions

Key Points

Discussion

Implication

Cite This Study

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