Key result
Deep mutational scanning and CRISPR editing characterize viral proteins to guide vaccine and therapeutic development.
Deep mutational scanning and CRISPR-based genome editing are powerful tools for understanding viral evolution and informing future vaccine and therapeutic development.
Recent advancements in synthetic biology and sequencing technologies have revolutionized the ability to manipulate viral genomes with unparalleled precision. This review focuses on two powerful methodologies: deep mutational scanning and CRISPR-based genome editing, that enable comprehensive mutagenesis and detailed functional characterization of viral proteins. These approaches have significantly deepened our understanding of the molecular determinants driving viral evolution and adaptation. Furthermore, we discuss how these advances provide transformative insights for future vaccine development and therapeutic strategies.
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Paz et al. (2025) conducted a review in Viral evolution and adaptation. Deep mutational scanning and CRISPR-based genome editing was evaluated. Deep mutational scanning and CRISPR-based genome editing enable comprehensive mutagenesis and detailed functional characterization of viral proteins to guide vaccine and therapeutic development.
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