Key result
Incubation of rat glomerulosa cells with a monoclonal antibody to the AT1 receptor concentrated the receptor on the cell surface and stimulated aldosterone output and IP3 generation.
Population
rat adrenal zona glomerulosa cells
Design
Preclinical
Authors
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Does not support clinical use of AT1 antibodies; leaves open whether receptor surface retention drives human aldosterone secretion.
In rat glomerulosa cells, retention of the AT1 receptor at the plasma membrane is sufficient to drive maximal steroidogenesis via IP3 generation, independent of PKC activation.
Vinson et al. (1995) studied this question. Monoclonal antibody (6313/G2) to the AT1 receptor was evaluated on Receptor internalisation, aldosterone output, IP3 generation, and PKC activation. Incubation of rat glomerulosa cells with a monoclonal antibody to the AT1 receptor concentrated the receptor on the cell surface and stimulated aldosterone output and IP3 generation.
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