This research demonstrates a modified fluorimetric method for estimating catecholamines in urine, suggesting improved accuracy in detection.
Some modifications in the fluorimetric method of E uler and F loding are described. The urine sample, usually 25 ml, is adjusted to pH 8.2–8.3 and passed through a column of aluminium oxide. The catechols are eluted with acetic acid, neutralized with ammonia to pH 6.2–6.3, oxidized with potassium ferricyanide and transformed to stable lutines with alkali and ascorbic acid. The fluorescence of the lutines is read with two filter sets and the amounts of adrenaline and noradrenaline computed.
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Euler et al. (1959) studied this question.
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