Key result
Resveratrol induced a brown fat-like phenotype in 3T3-L1 adipocytes by increasing the expression of UCP1, PPAR-γ, and PGC-1α, partly via activation of the mTOR pathway.
Why the study?
Browning of white adipose tissues is recognized as a novel way to combat obesity, but the mechanism of browning induced by resveratrol required exploration.
Does resveratrol induce a brown fat-like phenotype in 3T3-L1 adipocytes?
Does resveratrol induce a brown fat-like phenotype in 3T3-L1 adipocytes?
Resveratrol induces a brown-like adipocyte phenotype in 3T3-L1 cells partly via the mTOR pathway, suggesting potential as a dietary agent to combat obesity.
May support resveratrol for obesity via adipocyte browning; leaves open human translation from this in vitro evidence.
BACKGROUND: Browning of white adipose tissues (WAT) is recognized as a novel way to combat obesity and its related comorbidities. Thus, a lot of dietary agents contributing to browning of WAT have been identified. OBJECTIVE: In this study, we try to explore the mechanism of the browning of WAT induced by resveratrol (Res) in 3T3-L1 adipocytes. METHODS: The levels of cell viability and lipid accumulation were evaluated under different concentrations of Res. Cell signaling pathway analysis was performed to investigate the possible mechanisms of the WAT browning effect of Res in 3T3-L1 cells. RESULTS: We found that Res induced the brown fat-like phenotype by activating protein expressions of brown adipocyte-specific markers, such as peroxisome proliferator-activated receptor gamma (PPAR-γ), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), and uncoupling protein 1 (UCP1). Besides, Res reduced lipid accumulation, as shown by Oil Red O staining. The increased small lipid droplets implied that Res-treated 3T3-L1 adipocytes had some features of brown adipocytes. The brown fat-like phenotype in 3T3-L1 adipocytes induced by Res was possibly mediated by activation of mammalian target of rapamycin (mTOR), as brown adipocyte-specific markers were decreased by rapamycin, an inhibitor of mTOR and the MHY1485 treatment, an activator of mTOR, showed the similar effect of Res on browning markers. CONCLUSIONS: Res induced brown-like adipocyte phenotype in 3T3-L1 adipocytes partly via mTOR pathway, which provided new insights into the utilization of Res to prevent obesity and related comorbidities.
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Liu et al. (2020) studied Obesity (in vitro model). Resveratrol vs. Control (0.08% DMSO) was evaluated on Expression of brown adipocyte-specific markers (PPAR-γ, PGC-1α, UCP1) (p=<0.05). Resveratrol induced a brown fat-like phenotype in 3T3-L1 adipocytes by increasing the expression of UCP1, PPAR-γ, and PGC-1α, partly via activation of the mTOR pathway.
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