What question did this study set out to answer?

The aim was to identify optimal methods for thawing, reconstituting, and storing cryopreserved MSCs to enhance their viability and stability for clinical use.

April 20, 2023Regenerative TherapyOpen Access

Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants

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Authors

RARasmus Roost Aabling TAToke Alstrup EKEmma Mader Kjær

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Overview

Randomized trial identifies effective reconstitution methods for enhancing MSC viability and stability, suggesting improvements for cellular therapies.

Key Points

The aim was to identify optimal methods for thawing, reconstituting, and storing cryopreserved MSCs to enhance their viability and stability for clinical use.
Expanded human adipose tissue-derived MSCs using human platelet lysate and cryopreserved with DMSO.
Tested isotonic solutions with/without human serum albumin for thawing and storage.
Evaluated total MSC numbers and viability using flow cytometry with 7-AAD staining.
Reconstitution in protein-free solutions led to >50% MSC loss; protein presence was essential for thawing.
Storage in standard culture medium resulted in >40% cell loss and <80% viability after 1 hour.
Isotonic saline demonstrated >90% viability with no cell loss for 4 hours when MSCs were diluted properly.

Cite This Study

Aabling et al. (2023) studied this question.

synapsesocial.com/papers/6a1ec8a1bf2a5d44faaf5136 https://doi.org/10.1016/j.reth.2023.03.006

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April 20, 2023Regenerative TherapyOpen Access

Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants

View Full Paper

Ask AI

Bookmark

Authors

RARasmus Roost Aabling TAToke Alstrup EKEmma Mader Kjær

Discussion

Member takes

Overview

Randomized trial identifies effective reconstitution methods for enhancing MSC viability and stability, suggesting improvements for cellular therapies.

Key Points

The aim was to identify optimal methods for thawing, reconstituting, and storing cryopreserved MSCs to enhance their viability and stability for clinical use.
Expanded human adipose tissue-derived MSCs using human platelet lysate and cryopreserved with DMSO.
Tested isotonic solutions with/without human serum albumin for thawing and storage.
Evaluated total MSC numbers and viability using flow cytometry with 7-AAD staining.
Reconstitution in protein-free solutions led to >50% MSC loss; protein presence was essential for thawing.
Storage in standard culture medium resulted in >40% cell loss and <80% viability after 1 hour.
Isotonic saline demonstrated >90% viability with no cell loss for 4 hours when MSCs were diluted properly.

Cite This Study

Aabling et al. (2023) studied this question.

synapsesocial.com/papers/6a1ec8a1bf2a5d44faaf5136 https://doi.org/10.1016/j.reth.2023.03.006

Also Consider

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