Chronic cold exposure reprograms feeding-regulated LPL activity in white adipose tissues through hepatic ANGPTL3 and ANGPTL8

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  Figure 1. Chronic cold exposure reverses feeding-regulated LPL activity in scWAT through the hepatic insulin-ANGPTL8-ANGPTL3 axis. (a) Representative H&E staining of scWAT in mice. Scale bar, 100 μm. Mice were housed at RT or at 4°C (cold) for three months and samples were collected at fasting (fasted) or postprandial state (refed) (n = 6 mice/group, male, 20 weeks old). (b) Tissue LPL activity of mice housed at RT or at 4°C (cold) for 12 weeks (n = 6−8 mice/group, male, 20 weeks old). BAT, brown adipose tissue. (c) Hepatic Angptl8 mRNA levels in mice housed at RT or at 4°C (cold) for three months (n = 5−6 mice/group, male, 20 weeks old). Samples were collected at fasting (fasted) or postprandial state (refed). (d) Representative immunoblots of indicated proteins in the scWAT of mice used in (a). FL, C, and N represent full-length, C-terminal, and N-terminal of ANGPTL3, respectively. VCL, Vinculin. (e) Representative immunoblots of ANGPTL3 in the scWAT of Angptl8−/− mice and littermate control WT mice. Mice were housed at RT or at 4°C (cold) for three months and samples were collected at postprandial state (n = 3 mice/group, female, 20 weeks old). (f) Tissue LPL activity in Angptl3−/− mice…