Serum ANGPTL4 levels positively correlated with free fatty acids (P=0.044) and waist-hip ratio (P=0.016), while low ANGPTL3 associated with decreased HDL and increased triglycerides.
Cross-Sectional (n=250)
What are the normal serum levels of ANGPTL3 and ANGPTL4, and how do they correlate with lipid and carbohydrate metabolism in a general population sample?
Newly developed ELISAs demonstrate that serum ANGPTL3 and ANGPTL4 levels correlate with specific lipid parameters and anthropometric measures, supporting their role in human energy metabolism.
We have developed and validated quantitative ELISAs for human angiopoietin-like (ANGPTL)3 and 4 and correlated their serum levels with parameters of lipid and carbohydrate metabolism. For this study, we used a random subsample of the Health 2000 Health Examination Survey consisting of 125 men and 125 women, aged 30-94 years. The anthropometric and biochemical parameters of subjects were characterized in detail. ANGPTL 3 and 4 levels were determined using the developed ELISAs. The intra- and inter-assay coefficients of variation for the assays were less than 15%. The average serum concentration of ANGPTL3 was 368 +/- 168 ng/ml (mean +/- SD) and for ANGPTL4 it was 18 +/- 23 ng/ml (mean +/- SD). ANGPTL4 serum levels displayed high variability between individuals ranging from 2 to 158 ng/ml. In post-heparin plasma, both ANGPTL 3 and 4 were increased. Low levels of ANGPTL3 were associated with decreased HDL-cholesterol and increased triglyceride levels. ANGPTL4 levels were positively correlated with FFAs (P = 0.044) and waist-hip ratio (P = 0.016). The developed ELISAs will be important tools to clarify the role of ANGPTL 3 and 4 in human energy metabolism and partitioning of triglycerides between sites of storage (adipose tissue) and oxidation (skeletal and cardiac muscle).
Robciuc et al. (Wed,) conducted a cross-sectional in General population (n=250). Serum ANGPTL3 and ANGPTL4 levels was evaluated on Correlation with parameters of lipid and carbohydrate metabolism. Serum ANGPTL4 levels positively correlated with free fatty acids (P=0.044) and waist-hip ratio (P=0.016), while low ANGPTL3 associated with decreased HDL and increased triglycerides.