January 21, 1997Circulation
Upregulation of Vascular Angiotensin II Receptor Gene Expression by Low-Density Lipoprotein in Vascular Smooth Muscle Cells
Key result
Incubation of cultured rat vascular smooth muscle cells with 100 µg/mL LDL significantly increased AT1 receptor mRNA to approximately 250% of control levels.
Population
Cultured rat vascular smooth muscle cells
Comparison
Incubation with low-density lipoprotein at doses… vs Control cells pretreated with vehicle
Design
Preclinical
Follow-up
up to 24 hours
Authors
Discussion
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Member takes
Implication
No immediate clinical implications; leaves open LDL-AT1 receptor interactions in human vascular disease.
Structured PICO
P
Population
Cultured rat vascular smooth muscle cells.
I
Intervention
Incubation with low-density lipoprotein (LDL) at doses up to 100 micrograms/mL
C
Comparator
Control cells pretreated with vehicle
O
Outcome
AT1 receptor mRNA levels and cell surface-associated AT1 receptorssurrogate
Main Result
Absolute Event Rate: 250% vs 100%
LDL upregulates AT1 receptor gene expression in vascular smooth muscle cells via posttranscriptional mRNA stabilization, enhancing the functional response to angiotensin II.
Cite This Study
Nickenig et al. (1997) studied this question. Low-Density Lipoprotein (LDL) vs. vehicle/control was evaluated on AT1 receptor mRNA levels. Incubation of cultured rat vascular smooth muscle cells with 100 µg/mL LDL significantly increased AT1 receptor mRNA to approximately 250% of control levels.