A reversal in relative mobility of the two large subunits of brine shrimp (Na⁺ + K⁺)‐adenosinetriphosphatase

The two large subunits of brine shrimp Na,K-ATPase can be resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at neutral pH and at acidic pH. These subunits appear to reverse their positions on the gel relative to each other when resolved at acidic pH relative to neutral pH. The migration of both subunits is apparently affected by charge, even in the presence of 2.5% sodium dodecyl sulfate.