What question did this study set out to answer?

To identify the molecular identity of the zebrafish astray gene and determine its functional requirement during vertebrate retinal axon guidance.

April 20, 2001Science

astray , a Zebrafish roundabout Homolog Required for Retinal Axon Guidance

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To identify the molecular identity of the zebrafish astray gene and determine its functional requirement during vertebrate retinal axon guidance.
Examined retinal axon projection and pathfinding phenotypes in zebrafish astray mutants.
Performed eye transplantation assays to determine the tissue-specific requirement of astray function.
Cloned the astray locus and analyzed its expression in extending retinal ganglion cells.
Mutations in astray caused marked anterior-posterior pathfinding defects, excessive midline crossing, and defasciculation along the retinal projection.
Eye transplantation demonstrated that astray acts cell-autonomously within the eye rather than in surrounding brain tissue.
Identified astray as the zebrafish roundabout receptor robo2, which retinal ganglion cells express during axon extension.

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Implication

Genetic analysis reveals that robo2 regulates retinal axon pathfinding and midline crossing in zebrafish, highlighting its essential role in vertebrate visual system wiring.

Cite This Study

Fricke et al. (2001) studied this question.

synapsesocial.com/papers/6a1efc1c08df8bb755b303cd https://doi.org/10.1126/science.1059496

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Implication

Genetic analysis reveals that robo2 regulates retinal axon pathfinding and midline crossing in zebrafish, highlighting its essential role in vertebrate visual system wiring.

astray , a Zebrafish roundabout Homolog Required for Retinal Axon Guidance

Key Points

Discussion

Implication

Cite This Study

astray , a Zebrafish roundabout Homolog Required for Retinal Axon Guidance

Key Points

Discussion

Implication

Cite This Study

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