What is the clinical evidence from this study?

Study design: Other. Population: Healthy isolated cardiomyocytes (n=17). Intervention: Active contraction (1 Hz electrical stimulation) vs. Quiescence. Primary outcome: Mitochondrial deformation anisotropy.

July 11, 2011PLoS ONEOpen Access

Analysis of Mitochondrial 3D-Deformation in Cardiomyocytes during Active Contraction Reveals Passive Structural Anisotropy of Orthogonal Short Axes

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Key result

During active contraction of isolated cardiomyocytes, mitochondria exhibited significant deformation anisotropy between their orthogonal short-axes, revealing distinct spatial stiffness characteristics of the cytoskeleton.

Population

Isolated cardiomyocytes (in vitro model)

Comparison

Electrical stimulation to induce active… vs Quiescent state (rest) or control cells

Design

Preclinical

Authors

YYYael YanivTechnion – Israel Institute of Technology MJMagdalena JuhaszovaElectrophysiology SWSu WangShenyang Pharmaceutical University

Discussion

Member takes

Implication

Does not inform clinical practice; extends cytoskeletal models in isolated cells but leaves translation open.

Structured PICO

Population

17 isolated cardiomyocytes from healthy adult rats analyzed for mitochondrial 3D-deformation during active contraction.

Intervention

Electrical stimulation (1 Hz) to induce active contraction, and exposure to diazoxide (Dz, 30 mM)

Comparator

Quiescent state (rest) or control cells

Outcome

Mitochondrial 3D-deformation (length, width, and calculated thickness) and sarcomere length during contraction-relaxation cyclessurrogate

A novel method reveals that mitochondria in isolated cardiomyocytes undergo anisotropic 3D deformation during active contraction, reflecting the passive structural anisotropy of the cellular cytoskeleton.

Limitations

Mitochondrial 3D deformations were calculated based on measurements along two axes only, assuming deformed-spherical/ellipsoidal geometry.
The Fourier transform method yields average behavior, meaning rare events in single mitochondria are not detectable.
The perinuclear pool of mitochondria was not examined.

Cite This Study

Yaniv et al. (2011) studied Healthy isolated cardiomyocytes (n=17). Active contraction (1 Hz electrical stimulation) vs. Quiescence was evaluated on Mitochondrial deformation anisotropy. During active contraction of isolated cardiomyocytes, mitochondria exhibited significant deformation anisotropy between their orthogonal short-axes, revealing distinct spatial stiffness characteristics of the cytoskeleton.

synapsesocial.com/papers/6a1efca416ec2d13766b6086 https://doi.org/10.1371/journal.pone.0021985

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Analysis of Mitochondrial 3D-Deformation in Cardiomyocytes during Active Contraction Reveals Passive Structural Anisotropy of Orthogonal Short Axes

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Analysis of Mitochondrial 3D-Deformation in Cardiomyocytes during Active Contraction Reveals Passive Structural Anisotropy of Orthogonal Short Axes

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider