What question did this study set out to answer?

Identify specific cell-surface marker signatures to enable the isolation and enrichment of pure neural stem cells, glia, and neurons derived from human pluripotent stem cells using fluorescence-activated cell sorting.

March 2, 2011PLoS ONEOpen Access

Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells

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Authors

SYShauna H. YuanUniversity of Minnesota JMJody MartinBecton Dickinson (United States)JEJeanne EliaBioLegend (United States)

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Implication

In vitro study reveals surface marker panels for sorting neural stem cells, glia, and neurons, indicating efficient enrichment strategies.

Key Points

Identify specific cell-surface marker signatures to enable the isolation and enrichment of pure neural stem cells, glia, and neurons derived from human pluripotent stem cells using fluorescence-activated cell sorting.
Conducted an unbiased FACS- and image-based immunophenotyping screen using 190 antibodies against surface markers on naive human embryonic stem cells and neural differentiation derivatives.
Isolated candidate cell populations using prospective marker panels from human embryonic stem cell and induced pluripotent stem cell neural induction cultures.
Evaluated the viability, proliferation, marker expression, electrophysiological properties, and differentiation potential of isolated cells in vitro and in vivo.
Identified and purified a viable neural stem cell population characterized by CD184(+)/CD271(-)/CD44(-)/CD24(+) that propagated across multiple passages and differentiated into both neurons and glia.
Purified functional neurons defined by CD184(-)/CD44(-)/CD15(LOW)/CD24(+), which fired action potentials and expressed mature subtype-specific markers.
Enriched a mitotic glial population defined by CD184(+)/CD44(+) capable of differentiating into mature GFAP-expressing astrocytes in vitro and in vivo.

Cite This Study

Yuan et al. (2011) studied this question.

synapsesocial.com/papers/6a1f015b1baba023eb6b6351 https://doi.org/10.1371/journal.pone.0017540

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Implication

In vitro study reveals surface marker panels for sorting neural stem cells, glia, and neurons, indicating efficient enrichment strategies.

Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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