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October 29, 2013American Journal of TransplantationOpen Access

A Comprehensive and Quantitative Analysis of the Major Specificities in Rabbit Antithymocyte Globulin Preparations

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Authors

IPIrene PopowInstitut für ökologische WirtschaftsforschungJLJudith LeitnerMedical University of ViennaKGKatharina Grabmeier‐PfistershammerMedical University of Vienna

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Implication

Preclinical study reveals distinct antigen targets between clinical rabbit antithymocyte globulin formulations, indicating mechanisms governing their differential immunosuppressive effects.

Key Points

  • To identify and quantitatively map the primary human antigen targets recognized by two clinical rabbit antithymocyte globulin preparations, ATG-Fresenius and Thymoglobulin.
  • Screened human target antigens using retroviral expression cloning to isolate previously uncharacterized antithymocyte globulin (ATG) specificities.
  • Engineered cell lines with high antigen expression levels to quantify the precise antibody titers directed against individual targets in ATG-Fresenius and Thymoglobulin.
  • Selectively absorbed antibodies against the T cell receptor complex, CD2, and CD28 to determine their requirement for T-cell activation and forkhead box P3 induction.
  • Identified novel antigens that, together with previously known targets, account for the vast majority of human cell-directed antibodies present in rabbit ATG products.
  • Observed distinct antigen specificity and quantitative antibody concentration profiles between ATG-Fresenius and Thymoglobulin preparations.
  • Demonstrated that antibody depletion against the T cell receptor complex, CD2, and CD28 completely abrogated ATG-mediated T-cell activation and forkhead box P3 expression.

Cite This Study

Popow et al. (2013) studied this question.

synapsesocial.com/papers/6a1f0cf252be8f5fb3e6c8bahttps://doi.org/10.1111/ajt.12514
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