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May 28, 2009Cardiovascular ResearchOpen Access

AMP-activated protein kinase confers protection against TNF-α-induced cardiac cell death

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Key result

Dexamethasone and metformin abrogated TNF-alpha-induced caspase-3 activation and apoptotic cell death in adult rat ventricular cardiomyocytes by stimulating AMPK.

Population

adult rat ventricular cardiomyocytes

Comparison

Dexamethasone or metformin incubated for 0-12 h vs TNF-alpha alone

Design

Preclinical

Follow-up

0-12 h

Authors

GKGirish KewalramaniNatural Sciences and Engineering Research CouncilPPPrasanth PuthanveetilMidwestern UniversityFWFang WangChangchun University of Science and Technology

Discussion

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Implication

No immediate clinical implications; leaves open AMPK-mediated protection against TNF-alpha apoptosis in human cardiomyocytes.

Structured PICO

P
Population
Adult rat ventricular cardiomyocytes incubated with dexamethasone, metformin, or TNF-alpha for 0-12 hours.
I
Intervention
Dexamethasone or metformin incubated for 0-12 h
C
Comparator
TNF-alpha alone
O
Outcome
TNF-alpha-induced apoptosis (evaluated by caspase-3 activity and Hoechst staining)surrogate

AMPK activation by dexamethasone or metformin protects adult rat ventricular cardiomyocytes against TNF-alpha-induced apoptosis by regulating Bad and mitochondrial apoptotic mechanisms.

Cite This Study

Kewalramani et al. (2009) studied TNF-alpha-induced apoptosis. AMPK activators (dexamethasone or metformin) vs. TNF-alpha alone was evaluated on TNF-alpha-induced cell damage (caspase-3 activity and Hoechst staining). Dexamethasone and metformin abrogated TNF-alpha-induced caspase-3 activation and apoptotic cell death in adult rat ventricular cardiomyocytes by stimulating AMPK.

synapsesocial.com/papers/6a1f1d560a1e4e63fe914e07https://doi.org/10.1093/cvr/cvp166

Topics

Bremelanotide
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