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5′‐(4‐Aminophenyl‐phosphoryl)‐uridine‐2′(3′)‐phosphate was synthesized and coupled to Sepharose by activation with cyanogen bromide. This conjugate was used as a specific adsorbent for purification of ribonuclease A by affinity chromatography. Totally reduced and oxidized RNase preparations which are enzymatically inactive were not adsorbed or retarded by the RNase‐specific column, while S‐protein is strongly retarded.
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Wilchek et al. (1969) studied this question.
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