Key result
A simple, robust PCR genotyping method based on primer competition was developed for mdx, mdx3cv, and mdx4cv mice, which could also be applied to other point-mutation models.
A new primer competition PCR method simplifies the genotyping of mdx, mdx3cv, and mdx4cv mouse models of Duchenne muscular dystrophy.
Offers no immediate clinical application in DMD; extends primer-competition PCR to mdx variants for preclinical research.
mdx, mdx3cv, and mdx4cv mice are among the most commonly used models for the study of Duchenne muscular dystrophy. Their disease is caused by point mutations in the dystrophin gene. Despite widespread use of these models, genotyping has not always been straightforward. Current methods require multiple polymerase chain reactions (PCRs), post-PCR manipulations, and/or special equipment/reagents. Herein we report a simple, robust PCR genotyping method based on primer competition. This approach could also be applied in genotyping other point-mutation models.
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Shin et al. (2010) studied Duchenne muscular dystrophy (mouse models). Primer competition polymerase chain reaction vs. Current methods (multiple PCRs, post-PCR manipulations) was evaluated on Genotyping of mdx, mdx3cv, and mdx4cv mice. A simple, robust PCR genotyping method based on primer competition was developed for mdx, mdx3cv, and mdx4cv mice, which could also be applied to other point-mutation models.
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