A new sequencing protocol accurately genotypes mdx mouse models, preventing false positives associated with standard PCR methods.
Supports accurate mdx mouse genotyping; leaves open translation to clinical DMD testing.
Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx, mdx(4cv), and mdx(5cv) mice. This method clearly distinguishes between wildtype, heterozygous, and mutant transcripts, and thereby time and money can be saved by avoiding false positives.
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Banks et al. (2010) studied this question.
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