Key result
Extracellular Ca2+ promoted nitric oxide generation and vasorelaxation via CaSR- and Gq/11 protein-mediated eNOS activation in human vascular endothelial cells.
Why the study?
This study was conducted to examine the possible involvement of the Ca2+-sensing receptor in nitric oxide production in human vascular endothelial cells.
Does extracellular Ca2+ promote nitric oxide production via the Ca2+-sensing receptor in human vascular endothelial cells?
Does extracellular Ca2+ promote nitric oxide production via the Ca2+-sensing receptor in human vascular endothelial cells?
Extracellular Ca2+ promotes nitric oxide generation and subsequent vasorelaxation via CaSR- and Gq/11 protein-mediated eNOS activation in vascular endothelial cells.
Does not support practice change; leaves open CaSR as therapeutic target in human vascular disease.
This study examined the possible involvement of Ca2+-sensing receptor (CaSR) in nitric oxide (NO) production in human vascular endothelial cells. Extracellular Ca2+ elevated the intracellular Ca2+ concentration, the endothelial NO synthase (eNOS) phosphorylation level, and NO release from the cells. These responses were inhibited by a CaSR antagonist and a Gq/11 protein inhibitor. Application of an endothelial cell suspension induced vasorelaxation in isolated rat thoracic aorta precontracted by phenylephrine. Adding an NO scavenger to the organ bath abolished this vasorelaxation response. These results suggest that extracellular Ca2+ promotes NO generation via CaSR- and Gq/11 protein-mediated eNOS activation.
No takes yet. Share an insight, caveat, or question.
Horinouchi et al. (2019) studied this question. Extracellular Ca2+ vs. CaSR antagonist and Gq/11 protein inhibitor was evaluated on Nitric oxide (NO) production and vasorelaxation. Extracellular Ca2+ promoted nitric oxide generation and vasorelaxation via CaSR- and Gq/11 protein-mediated eNOS activation in human vascular endothelial cells.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: