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September 1, 1995AJP Cell Physiology

Regulation of Ca(2+)-dependent nitric oxide synthase in bovine aortic endothelial cells

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Population

Bovine aortic endothelial cells

Comparison

Stimulation with Ca-mobilizing agonists and… vs Basal/control levels

Design

Preclinical

Authors

BBBarbara J. BuckleyThompson Rivers UniversityZMZermeena MirzaDuke UniversityAWA. Richard WhortonDuke Medical Center

Discussion

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Implication

Findings in bovine cells remain preclinical; leaves open translation to human endothelial therapies.

Structured PICO

P
Population
Bovine aortic endothelial cells
I
Intervention
Stimulation with Ca(2+)-mobilizing agonists (bradykinin, ATP, A-23187, thapsigargin) and exposure to NO gas or S-nitrosoglutathione
C
Comparator
Basal/control levels
O
Outcome
Kinetics of NO formation and intracellular Ca2+ concentration ([Ca2+]i)surrogate

In bovine aortic endothelial cells, Ca2+-mobilizing agonists stimulate NO production, which is dependent on extracellular L-arginine and intracellular Ca2+, and is subject to negative feedback by NO itself.

Cite This Study

Buckley et al. (1995) studied this question.

synapsesocial.com/papers/6a915f91fbd14437d06f2f50https://doi.org/10.1152/ajpcell.1995.269.3.c757
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Calmodulin-dependent endothelium-derived relaxing factor/nitric oxide synthase activity is present in the particulate and cytosolic fractions of bovine aortic endothelial cells.1991 · 594 citations
  2. 2Phosphorylation and subcellular translocation of endothelial nitric oxide synthase.1993 · 325 citations
  3. 3Effects of L-arginine on forearm vessels and responses to acetylcholine.1992 · 104 citations
  4. 4A perfusion system for the long term study of macrophage activation1992 · 25 citations
  5. 5Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells.1991 · 986 citations