Key result
Large and long shear stress significantly increased nitric oxide synthesis and release in both normal HUVECs and those exposed to supraphysiologic glucose (p<0.05).
Why the study?
To analyze how the magnitude and duration of shear stress affect nitric oxide synthesis and release in normal HUVEC cultures and those exposed to supraphysiologic glucose.
Does large, prolonged shear stress improve nitric oxide synthesis and release in endothelial cells exposed to supraphysiologic glucose?
Does large, prolonged shear stress improve nitric oxide synthesis and release in endothelial cells exposed to supraphysiologic glucose?
Large and prolonged shear stress can improve nitric oxide synthesis and release in endothelial cells, even in the presence of endothelial dysfunction induced by high glucose.
Answers are grounded in this paper and cite their sources.
Hypothesis-generating for shear stress in hyperglycemic endothelium; requires in vivo validation before any clinical consideration.
The purpose of this study is to analyze the magnitude and duration of shear stress in normal endothelial cell (HUVECs) cultures and those exposed to supraphicological glucose which affected synthesis and release of NO detected by bioassay techniques. The results of this study concluded that large shear stress applied to endothelial cell culture for a long time (± 15 minutes) will increase the synthesis and release of NO. In normal endothelial cell culture a large shear stress exposure in a long time can increase NO synthesis and release very significantly compared to low shear stress (6 dyne/cm2 for 5 minutes). Likewise, endothelial cell culture which was exposed to 22 mM glucose for 7 days, giving a large amount of shear stress for a long time (15 minutes) could increase NO synthesis and release, although it was far compared to normal endothelial cell culture. It can be said that the synthesis and release of NO in endothelial dysfunction can be improved through exposure to large and long-standing shear stress.
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Wahyudi et al. (2019) studied Endothelial dysfunction (in vitro). Shear stress vs. Normal conditions / low shear stress was evaluated on Nitric oxide (NO) synthesis and release (p=<0.05). Large and long shear stress significantly increased nitric oxide synthesis and release in both normal HUVECs and those exposed to supraphysiologic glucose (p<0.05).
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