What question did this study set out to answer?

To evaluate whether natural killer (NK) cells from HIV-infected individuals produce CC-chemokines and block HIV-1 entry and replication in autologous cells.

July 1, 1998Journal of Clinical InvestigationOpen Access

Natural killer cells from human immunodeficiency virus (HIV)-infected individuals are an important source of CC-chemokines and suppress HIV-1 entry and replication in vitro.

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To evaluate whether natural killer (NK) cells from HIV-infected individuals produce CC-chemokines and block HIV-1 entry and replication in autologous cells.
Isolated NK cells from HIV-infected individuals and measured mRNA and secretion of MIP-1alpha, MIP-1beta, and RANTES under baseline, IL-2/IL-15 stimulation, target cell lysis, and CD16 cross-linking conditions.
Quantified HIV-1 suppression in cocultures of autologous CD8/NK-depleted PBMCs and tested the capacity of NK supernatants to inhibit MT-2-negative HIV-1 (strain BaL) entry into CD4(+)CCR5(+) PM-1 cells, with and without chemokine-neutralizing antibodies.
CD16 cross-linked NK cells stimulated with IL-2 or IL-15 suppressed viral replication by 49% to 97% in autologous cocultures, performing at levels comparable to stimulated CD8(+) T cells.
Neutralizing antibodies against MIP-1alpha, MIP-1beta, and RANTES reversed NK-mediated viral suppression in 50% of tested patient samples, whereas CD8(+) T cell suppression was unaffected.
Supernatants from activated NK cells markedly blocked the entry of CCR5-dependent HIV-1 BaL into CD4(+)CCR5(+) target T cells.

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Implication

In vitro study reveals natural killer cells suppress HIV-1 replication via CC-chemokines in HIV-infected individuals, highlighting an innate mechanism against viral entry.

Cite This Study

Oliva et al. (1998) studied this question.

synapsesocial.com/papers/6a1f827252dcfdcb78cf2eda https://doi.org/10.1172/jci2323

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Implication

In vitro study reveals natural killer cells suppress HIV-1 replication via CC-chemokines in HIV-infected individuals, highlighting an innate mechanism against viral entry.

Natural killer cells from human immunodeficiency virus (HIV)-infected individuals are an important source of CC-chemokines and suppress HIV-1 entry and replication in vitro.

Key Points

Discussion

Implication

Cite This Study

Natural killer cells from human immunodeficiency virus (HIV)-infected individuals are an important source of CC-chemokines and suppress HIV-1 entry and replication in vitro.

Key Points

Discussion

Implication

Cite This Study

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