Key result
Interferon-alpha treatment increased p203 protein levels in murine fibroblasts, with the protein localizing to the nucleus.
The study successfully cloned the 203 cDNA and demonstrated that its encoded protein, p203, is interferon-inducible and localizes to the nucleus in murine cells.
Murine fibroblast data warrant cautious extrapolation to clinical interferon use; leaves open p203 relevance in human cells.
We report here the complete coding sequence of a 203 cDNA, a member of the interferon-inducible Ifi 200 gene family. By combining reverse-transcriptase PCR and rapid amplification of cDNA ends (RACE) techniques we have obtained a 3.8-kb cDNA corresponding to a 203 mRNA. When used as a probe in northern analysis, its 3' segment hybridized to a 3.8-kb interferon-inducible mRNA, whereas the 5'-end additionally hybridized to a less abundant interferon-inducible 1.8-kb mRNA. Nucleotide sequence analysis revealed that the two mRNAs share the 5'-untranslated region and the same open reading frame, which encodes a hydrophilic protein composed of 408 amino acids. The difference between them is due to a 3'-untranslated region extended by alternative polyadenylation site selection. Furthermore, 203 mRNA was found to be inducible by interferon-alpha in various murine cell lines. Using polyclonal antibodies raised against a segment specific for the 203 protein, we established that p203 protein levels increase on treatment with interferon-alpha in murine fibroblasts and that p203 is located in the nucleus.
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Gribaudo et al. (1997) studied this question. Interferon-alpha was evaluated on 203 mRNA and p203 protein expression. Interferon-alpha treatment increased p203 protein levels in murine fibroblasts, with the protein localizing to the nucleus.
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