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May 31, 2026Annual Report of The Kansai Plant Protection SocietyOpen Access

Validation of ELISA-based analysis method for residual oxolinic acid in ume, apricot, and those processed liqueurs

Shotaro Hirata Hidenobu Hoshi

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Key Points

The aim is to validate an ELISA-based method for detecting residual oxolinic acid in ume and apricot products.
Extracted oxolinic acid using methanol-based buffer.
Measured sample solutions through antigen-antibody reactions using a microplate reader.
Conducted recovery tests at concentrations of 30 μg/g and 0.01 μg/g to assess accuracy.
Recovery rates for ume and apricot liqueurs were 91% to 95% confirming adequate accuracy.
Precision results showed parallel accuracy ≤ 9% and within-lab accuracy ≤ 13%.
No measurement inhibition was observed in blank samples.

Implication

Validates ELISA method for measuring residual oxolinic acid in ume and apricot liqueurs, suggesting reliable testing for safety.

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Cite This Study

Hirata et al. (2026) studied this question.

synapsesocial.com/papers/6a1fc3a6dee9eb8c0dce51ff https://doi.org/10.4165/kapps.68.84

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