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June 3, 2026Environmental Pollution1 citationsOpen Access

Monsoon-Triggered Surges of Antibiotic Resistance Genes in Urban Indian Rivers Impacted by Untreated Wastewater

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KBKenyum BagraIndian Institute of Technology BHUHSHarshita SinghIndian Institute of Technology RoorkeeUKUli KlümperTechnische Universität Dresden

Key Points

  • The study aims to investigate the impact of monsoon rainfall on the levels of antibiotic resistance genes in urban rivers influenced by untreated wastewater.
  • Monitored antibiotic resistance genes and faecal indicators in water and sediment samples pre- and post-monsoon.
  • Analyzed the correlation between heavy metal concentrations and antibiotic resistance gene abundances.
  • Assessed the effects of untreated wastewater on resistance levels downstream of treatment plants.
  • ARG levels increased 1 to 3 orders of magnitude during monsoon compared to pre-monsoon conditions.
  • Sediment-associated ARG signals remained elevated post-monsoon, indicating their persistence.
  • No significant correlations were found between heavy metal concentrations and ARG abundances.

Abstract

), indicating persistent faecal contamination. Monsoon rainfall was associated with a consistent increase in faecal/anthropogenic indicators and ARGs, reaching 1 to 3 orders of magnitude higher relative abundance compared to pre-monsoon conditions. Post-monsoon, ARG levels in water generally declined toward pre-monsoon levels, whereas sediment-associated ARG signals remained elevated post- compared to pre-monsoon, indicating persistence in depositional reservoirs. Although heavy metal concentrations frequently reached ranges previously linked to co-selection, they showed no significant correlations with ARG abundances, identifying faecal pollution as the dominant driver of seasonal resistance dynamics. No consistent increases in ARGs or faecal markers were detected downstream of wastewater treatment plants, supporting widespread non-point source inputs. Overall, monsoon events act as major amplifiers of antibiotic resistance in severely impacted urban rivers by mobilising faecal contamination and counteracting dilution. Mitigation should prioritise reducing unmanaged sewage and solid waste inputs, particularly before and during monsoon periods.

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Cite This Study

Bagra et al. (2026) studied this question.

synapsesocial.com/papers/6a1fc64adee9eb8c0dce77c3https://doi.org/10.1016/j.envpol.2026.128468
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