What is the clinical evidence from this study?

Study design: RCT. Population: Chronic renal failure. Intervention: Antioxidant-fortified diet (vitamin E) vs. Regular diet (vitamin E, 40 U/Kg food). Primary outcome: Blood pressure, plasma malondialdehyde (MDA), tissue NO synthase (NOS) isoforms, tissue nitrotyrosine, and vascular tissue NO production.

What question did this study set out to answer?

The study aims to investigate the impact of chronic renal failure on nitric oxide inactivation and protein nitration due to reactive oxygen species, and to assess the effects of antioxidant therapy.

January 1, 2002HypertensionOpen Access

Enhanced Nitric Oxide Inactivation and Protein Nitration by Reactive Oxygen Species in Renal Insufficiency

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Key result

High-dose vitamin E ameliorated CRF-induced hypertension, improved vascular NO production, lowered tissue nitrotyrosine burden, and reversed downregulations of NOS isoforms in rats.

Why the study?

Does high-dose vitamin E antioxidant therapy improve hypertension and nitric oxide availability in a rat model of chronic renal failure?

Population

Male Sprague Dawley rats with induced chronic renal failure (5/6 nephrectomy) and sham-operated controls

Comparison

Antioxidant-fortified diet for 6 weeks vs Regular diet (vitamin E, 40 U/Kg food) for 6 weeks

Design

Preclinical, Rats were randomized to CRF and sham-operated controls and fed…

Follow-up

6 weeks

Authors

NVNosratola D. VaziriUniversity of Geneva ZNZhenmin NiXuzhou Medical College FOFariba OveisiUniversity of California, Irvine

Discussion

Member takes

Implication

Should not change clinical practice; hypothesis-generating for antioxidant therapy targeting NO pathways in CRF models.

Key Points

The study aims to investigate the impact of chronic renal failure on nitric oxide inactivation and protein nitration due to reactive oxygen species, and to assess the effects of antioxidant therapy.
Male Sprague Dawley rats (N=...) were randomized to chronic renal failure and sham-operated controls.
Rats were fed regular or antioxidant-fortified diets (vitamin E, 5000 U/Kg food) for 6 weeks.
Blood pressure, plasma malondialdehyde, tissue NO synthase isoforms, and tissue nitrotyrosine were measured to assess outcomes.
Chronic renal failure significantly increased blood pressure and tissue nitrotyrosine levels (elevated by approximately x%).
Antioxidant therapy reduced hypertension and improved vascular tissue NO production (specific metrics not provided).
Antioxidants reversed downregulated NOS isoforms but did not affect control group measurements.

Study Design

Type

RCT

Randomization

randomized

Structured PICO

Does high-dose vitamin E antioxidant therapy improve hypertension and nitric oxide availability in a rat model of chronic renal failure?

Population

Male Sprague Dawley rats with induced chronic renal failure (5/6 nephrectomy) or sham operation, followed for 6 weeks.

Intervention

Antioxidant-fortified diet (vitamin E, 5000 U/Kg food) for 6 weeks

Comparator

Regular diet (vitamin E, 40 U/Kg food) for 6 weeks

Outcome

Blood pressure, plasma malondialdehyde (MDA), tissue NO synthase (NOS) isoforms, tissue nitrotyrosine, and vascular tissue NO productionsurrogate

High-dose vitamin E ameliorates oxidative stress, enhances nitric oxide availability, and improves hypertension in a rat model of chronic renal failure.

Cite This Study

Vaziri et al. (2002) conducted an RCT in Chronic renal failure. Antioxidant-fortified diet (vitamin E) vs. Regular diet (vitamin E, 40 U/Kg food) was evaluated on Blood pressure, plasma malondialdehyde (MDA), tissue NO synthase (NOS) isoforms, tissue nitrotyrosine, and vascular tissue NO production. High-dose vitamin E ameliorated CRF-induced hypertension, improved vascular NO production, lowered tissue nitrotyrosine burden, and reversed downregulations of NOS isoforms in rats.

synapsesocial.com/papers/6a1fc7e9ce3cb5ba46e69a37 https://doi.org/10.1161/hy0102.100540

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Enhanced Nitric Oxide Inactivation and Protein Nitration by Reactive Oxygen Species in Renal Insufficiency

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Cite This Study

Enhanced Nitric Oxide Inactivation and Protein Nitration by Reactive Oxygen Species in Renal Insufficiency

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Cite This Study

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