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November 1, 2001PROTEOMICS

Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Ejvind Mørtz Thomas N. Krogh Henrik Vorum Angelika Görg

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Abstract

In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.

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Mørtz et al. (2001) studied this question.

synapsesocial.com/papers/6a1fc8a684dc2dcb25989771 https://doi.org/10.1002/1615-9861(200111)1:11<1359::aid-prot1359>3.0.co;2-q

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