Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley.

Thylakoid membrane polypeptides of wild type barley and the chlorina i 2 mutant, which lacks chlorophyll b, were analyzed by two-dimensional polyacrylamide gel electrophoresis.The mutant lacks o r is greatly deficient in polypeptides 14 (M,.= 27,000), 15 (Mr = 25,000), 20 (Mr = 22,000), 24 (Mr = 21,000), and 25 (Mr = 20,000) and contains reduced amounts of polypeptide 16 (Mr = 24,000).Polypeptides 14, 15, and 16 are major components of the light-harvesting chlorophyll a/b-protein complex (Burke,