Key result
Acute activation of protein kinase A by 8-BrcAMP inhibited NHE-3 activity by 39% in AP-1/NHE-3 cells, an effect likely mediated by phosphorylation of its cytoplasmic domain.
Effect estimate: 39% inhibition
Absolute Event Rate: 0.4% vs 0.65%
p-value: p=<0.01
Acute activation of PKA inhibits NHE-3 activity, an effect that is likely mediated by phosphorylation of its cytoplasmic domain.
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Hypothesis-generating for PKA-NHE-3 regulation in epithelial transport; leaves open relevance to cardiovascular or renal disease.
Moe et al. (1995) studied this question. Protein Kinase A activation (8-BrcAMP) vs. Control (no cAMP) was evaluated on NHE-3 activity (Na-dependent intracellular pH recovery) (39% inhibition, p=<0.01). Acute activation of protein kinase A by 8-BrcAMP inhibited NHE-3 activity by 39% in AP-1/NHE-3 cells, an effect likely mediated by phosphorylation of its cytoplasmic domain.
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