What question did this study set out to answer?

This research analyzes the phosphotransferase activity of fragments from avian sarcoma virus-transforming proteins, pp60src and pp90yes.

April 1, 1984Journal of Biological ChemistryOpen Access

Analysis of the catalytic domain of phosphotransferase activity of two avian sarcoma virus-transforming proteins.

View Full Paper

Ask AI

Bookmark

This research analyzes the phosphotransferase activity of fragments from avian sarcoma virus-transforming proteins, pp60src and pp90yes.
Proteolytic digestion of pp60src and pp90yes with trypsin, chymotrypsin, or thermolysin.
Assessment of phosphorylating activity on specific immunoglobulin and exogenous substrates.
Comparison of specific activities of protease-resistant fragments versus intact enzymes.
The 29,000-dalton fragment of pp60src showed greater specific activity than the intact enzyme.
Both pp60src and pp90yes fragments maintain strict specificity for tyrosine phosphorylation.
The fragments can function independently as phosphotransferases with enhanced stability against proteolysis.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Analysis shows phosphotransferase activity of proteolytic fragments in avian sarcoma virus proteins, suggesting functional independence and stability.

Cite This Study

Brugge et al. (1984) studied this question.

synapsesocial.com/papers/6a1fd646f65d30f3523e659c https://doi.org/10.1016/s0021-9258(17)43081-x

View Full Paper

Ask AI

Bookmark

Implication

Analysis shows phosphotransferase activity of proteolytic fragments in avian sarcoma virus proteins, suggesting functional independence and stability.

Analysis of the catalytic domain of phosphotransferase activity of two avian sarcoma virus-transforming proteins.

Key Points

Discussion

Implication

Cite This Study

Analysis of the catalytic domain of phosphotransferase activity of two avian sarcoma virus-transforming proteins.

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider