What question did this study set out to answer?

The aim is to investigate how anaerobic conditions affect protein synthesis in heart muscle.

April 1, 1971Journal of Biological ChemistryOpen Access

Regulation of Protein Synthesis in Heart Muscle

View Full Paper

Ask AI

Bookmark

Population

isolated rat heart model

Comparison

perfusion under anaerobic conditions vs aerobic hearts perfused under the same conditions

Design

Preclinical

Authors

LJLeonard S. JeffersonQueen's University EWE WolpertPenn State Milton S. Hershey Medical Center KGK. GigerGeisinger Medical Center

Discussion

Member takes

Implication

May warrant caution in cardiac ischemia; leaves open GTP/GMP pathways for mechanistic research in myocardial metabolism.

Key Points

The aim is to investigate how anaerobic conditions affect protein synthesis in heart muscle.
Perfused isolated rat hearts under anaerobic conditions.
Measured protein synthesis, myosin levels, and amino acid incorporation.
Analyzed changes in ribosomal subunits and polysomes.
Protein synthesis was inhibited, with myosin synthesis similarly affected.
Intracellular amino acid levels increased, but aspartic and glutamic acids decreased.
GTP levels dropped by 50% while GMP levels tripled in anaerobic hearts.

Structured PICO

Population

isolated rat heart model

Intervention

perfusion under anaerobic conditions

Comparator

aerobic hearts perfused under the same conditions

Outcome

synthesis of whole heart protein and myosinsurrogate

Anaerobic conditions reversibly inhibit protein synthesis in the isolated rat heart by blocking amino acid incorporation, associated with altered GTP/GMP levels.

Cite This Study

Jefferson et al. (1971) studied this question.

synapsesocial.com/papers/6a1fd6c8c2e11e39673e661e https://doi.org/10.1016/s0021-9258(19)77204-4

View Full Paper

Ask AI

Bookmark

Regulation of Protein Synthesis in Heart Muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Regulation of Protein Synthesis in Heart Muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider