Severe obesity was associated with 22 differentially expressed proteins in visceral adipose tissue compared to healthy controls (FDR < 0.05), reflecting metabolic and inflammatory alterations.
Cross-Sectional (n=63)
Severe obesity is associated with significant molecular alterations in the visceral adipose tissue proteome, including downregulation of mitochondrial enzymes and upregulation of inflammatory proteins.
valor p: p=FDR < 0.05
OBJECTIVE: Severe obesity poses a major public health concern due to its links with cardiometabolic complications and mortality. Visceral adipose tissue (VAT) plays a key role in these processes through distinct molecular features. This study aimed to characterize the VAT proteome of individuals with severe obesity and investigate its association with serum metabolic biomarkers. METHODS: A cross-sectional analysis was performed for 46 individuals with severe obesity undergoing metabolic bariatric surgery and 17 healthy controls undergoing elective abdominal surgery. VAT proteomes were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and serum metabolites were quantified using nuclear magnetic resonance-based metabolomics. RESULTS: LC-MS/MS identified 22 differentially expressed proteins (FDR < 0.05) in VAT with 12 downregulated and 10 upregulated in severe obesity. Downregulated proteins included mitochondrial enzymes involved in substrate metabolism and mitochondrial transmembrane transport. Circulating glucose, valine, and isoleucine correlated negatively with VAT mitochondrial transmembrane and electron transport proteins. Upregulated proteins were associated with inflammation, immune activation, oxidative stress, cytoskeletal remodeling, and protein turnover. CONCLUSIONS: These findings demonstrate significant molecular alterations in the VAT proteome associated with severe obesity, providing insights into the underlying mechanisms of metabolic disease. The differentially expressed proteins may serve as biomarkers or therapeutic targets for obesity-related complications. TRIAL REGISTRATION: ClinicalTrials.gov identifiers: NCT00793143 and NCT01373892.
Dadson et al. (Sun,) conducted a cross-sectional in Severe obesity (n=63). Severe obesity vs. Healthy controls was evaluated on Differentially expressed proteins in visceral adipose tissue (p=FDR < 0.05). Severe obesity was associated with 22 differentially expressed proteins in visceral adipose tissue compared to healthy controls (FDR < 0.05), reflecting metabolic and inflammatory alterations.