Clostridium sediminicola sp. nov., a crude oil aggregation-forming anaerobic bacterium isolated from marine sediment

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An erratum of this article has been published full details can be found at https://doi.org/10.1099/ijsem.0.006671 A crude oil aggregation-forming, strictly anaerobic, Gram-stain-positive, spore-forming, rod-shaped, motile and mesophilic bacterium, named strain SH18-2 T , was isolated from marine sediment near Sado Island in the Sea of Japan. The temperature, salinity and pH ranges of this strain for the growth were 15–40 °C (optimum 35 °C), 0.5–6.0% NaCl (w/v; optimum 2.0%) and pH 6.0–8.0 (optimum 7.5), respectively. A phylogenetic analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Clostridium . It was most closely related to Clostridium grantii A-1 T (97.02% homology). The draft genome size was 5616089 bp, with a G+C content of 29.7 mol%. The digital DNA–DNA hybridization values determined using the Genome-to-Genome Distance Calculator and the average nucleotide identity between this strain and C. grantii A-1 T were 20.30 and 78.24%, respectively. Whole-organism hydrolysates of strain SH18-2 T contained meso- diaminopimelic acid; the major fatty acid was C 16:0, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipid and three unidentified polar lipids. From the results of genotypic, phenotypic and chemotaxonomic analyses, SH18-2 T (=NBRC 116030 T =DSM 115762 T ) is the type strain and represents a novel species of the genus Clostridium , and the name Clostridium sediminicola sp. nov. is proposed.