April 1, 1997Journal of Biological ChemistryOpen Access

Characterization of the Human Thrombopoietin Gene Promoter

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: Promoter fragment-luciferase reporter gene constructs and E4TF1 co-expression. Primary outcome: TPO gene expression and promoter activity.

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Key result

The Ets family transcription factor E4TF1(GABPalpha/beta) is required for high level expression of the human thrombopoietin gene in liver cells.

Population

Human liver cell lines (PLC and HepG2)

Design

Preclinical

Authors

TKTakumi KamuraNagoya University HHHiroshi HandaTokyo Medical University NHNaotaka HamasakiKurume University

Discussion

Member takes

Implication

E4TF1 regulation of thrombopoietin expression is hypothesis-generating; leaves open translational relevance in humans.

Structured PICO

Population

Human liver cell lines (PLC and HepG2)

Intervention

Promoter fragment-luciferase reporter gene constructs containing 5'-truncated sequences or site-directed mutations; co-expression of E4TF1-60 and E4TF1-53/47

Outcome

TPO gene promoter activity and expressionsurrogate

Identifies the Ets family transcription factor E4TF1 as essential for high-level expression of the human thrombopoietin gene in the liver.

Cite This Study

Kamura et al. (1997) studied this question. Promoter fragment-luciferase reporter gene constructs and E4TF1 co-expression was evaluated on TPO gene expression and promoter activity. The Ets family transcription factor E4TF1(GABPalpha/beta) is required for high level expression of the human thrombopoietin gene in liver cells.

synapsesocial.com/papers/6a2004b26bd9e57b9265add5 https://doi.org/10.1074/jbc.272.17.11361

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April 1, 1997Journal of Biological ChemistryOpen Access

Characterization of the Human Thrombopoietin Gene Promoter

View Full Paper

Ask AI

Bookmark

Key result

The Ets family transcription factor E4TF1(GABPalpha/beta) is required for high level expression of the human thrombopoietin gene in liver cells.

Population

Human liver cell lines (PLC and HepG2)

Design

Preclinical

Authors

TKTakumi KamuraNagoya University HHHiroshi HandaTokyo Medical University NHNaotaka HamasakiKurume University

Discussion

Member takes

Implication

E4TF1 regulation of thrombopoietin expression is hypothesis-generating; leaves open translational relevance in humans.

Structured PICO

Population

Human liver cell lines (PLC and HepG2)

Intervention

Promoter fragment-luciferase reporter gene constructs containing 5'-truncated sequences or site-directed mutations; co-expression of E4TF1-60 and E4TF1-53/47

Outcome

TPO gene promoter activity and expressionsurrogate

Identifies the Ets family transcription factor E4TF1 as essential for high-level expression of the human thrombopoietin gene in the liver.

Cite This Study

synapsesocial.com/papers/6a2004b26bd9e57b9265add5 https://doi.org/10.1074/jbc.272.17.11361

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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