Key result
Polymerization into F-actin and binding to myosin subfragment-1 protects existing protease cleavage sites on G-actin while exposing a new cleavage site (Arg39-His40) for ArgC protease.
Population
G-actin, F-actin, G-actin-S1(A2) and F-actin-S1(A2) complexes
Comparison
Protease attack vs Comparative basis between different actin states
Design
Preclinical
Authors
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No immediate clinical implications; leaves open translation of actin conformational changes to human cardiac models.
The study provides structural evidence for the mechanism of S1-induced polymerization of G-actin by demonstrating specific conformational changes in subdomain-2.
Fievez et al. (1993) studied this question. Polymerization and myosin subfragment-1 binding vs. G-actin was evaluated on Susceptibility of subdomain-2 of actin to different proteases. Polymerization into F-actin and binding to myosin subfragment-1 protects existing protease cleavage sites on G-actin while exposing a new cleavage site (Arg39-His40) for ArgC protease.
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