Characterization of the major polypeptides of the rat liver nuclear envelope.

Rat liver nuclear envelopes have been isolated using a newly developed method.Purified nuclei were digested with DNase I and RNase A and then extracted with 1.6 M NaCl in the presence of a reducing agent.This method, which is rapid and simple, yielded predominantly intact nuclear envelope spheres.After correction for phospholipid recovery (40-50%), these nuclear envelopes contained 13, 3, and 4 % of the total