14 C dating of bone has been unreliable in comparison with more stable materials such as wood or charcoal. Attempts have been made to use various components or fractions isolated from the raw bone sample; these include dilute acid soluble, dilute acid insoluble, collagen, and gelatin, as well as alkali soluble and insoluble fractions of burned bone, and carbonate or apatite fractions of organic-poor bone. All of these fractions have yielded useful data in some cases, but no single method has proven suitable in all situations. The work reported here describes the isolation and purification of amino acids from the dilute acid insoluble fraction of bone collagen and parchment, with some preliminary experiments on amino acids from shell conchiolin.
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Gillespie et al. (1986) studied this question.
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