What question did this study set out to answer?

To classify cells into distinct cell cycle compartments based on DNA and RNA measurements.

September 1, 1980CytometryOpen Access

New cell cycle compartments identified by multiparameter flow cytometry

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To classify cells into distinct cell cycle compartments based on DNA and RNA measurements.
Utilized multiparameter flow cytometry to assess cellular DNA and RNA in over 40 different cell systems.
Measured DNA sensitivity to acid denaturation and cell incorporation of BUdR to differentiate compartments.
Subclassified cells into 12 distinct cycle compartments based on these parameters.
Identified quiescent cells with low RNA and high DNA sensitivity to denaturation, not incorporating BUdR.
Found two distinct G 1 phases: G 1A and G 1B, with progression from G 1A to G 1B as an exponential event.
Distinguished a new category of transitional cells with intermediate values of RNA and DNA sensitivity to denote their state between quiescence and active cycling.

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Randomized trial identifies distinct cell cycle compartments based on DNA and RNA measurements, indicating cellular states.

Darżynkiewicz et al. (1980) studied this question.

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