Separation of Transfer Ribonucleic Acids by Reverse Phase Chromatography

Numerous experimental techniques for the separation of transfer ribonucleic acids have been successful in preparing partially purified fractions of several specific t-RNAs. Many of the existing methods have depended upon the differential solubility of specific t-RNAs in complex two-phase systems (1-6) and the separation was achieved by means of counter-current extraction techniques. Column chromatography experiments using cellulose exchangers, (7,8) methylated albumin (9,10) or with solvent phases supported on inert material (11-13) have also shown partial separation of specific t-RNAs. Paper chromatographic procedures have produced partial resolutions of t-RNA.¹⁴ Methods involving chemical treatment of specific t-RNAs have been reported (15-19).