Effects of supplementing methionine hydroxy analogue in soybean meal and distiller's dried grain-based diets on the performance and nutrient retention of rainbow trout [Oncorhynchus mykiss(Walbaum)]

Two experiments were conducted to evaluate the effects of supplementing methionine hydroxy analogue (MHA) on the performance, body composition, and nutrition retention of rainbow trout. In experiment 1, a 2×4 factorial arrangement with two MHA supplemental levels (with and without) and four fish meal replacement levels (25%, 50%, 75%, and 100%, replaced with soybean meal (SBM) and wheat gluten) was used. A fish meal diet was included as a control. Results showed that fish meal replacement levels had significant effects on fish weight gain (WG), feed conversion ratio (FCR), body moisture, crude protein, crude fat, and ash (P<0.05). In experiment 2, a fish meal reference diet and seven other diets were made using SBM and distiller's dried grain with solubles supplemented with 0, 0, 0.55, 1.1, 1.65, 2.2, and 2.75 g MHA kg−1 diet to replace 50% of fish meal. WG, FCR, and apparent retention of crude protein and phosphorus were significantly improved in rainbow trout fed the diet containing 1.65 g MHA kg−1 diet compared with fish fed an equivalent diet without MHA.