What question did this study set out to answer?

To clone the metG gene from E. coli and analyze its product, methionyl-tRNA synthetase.

December 1, 1984Journal of BacteriologyOpen Access

Molecular cloning and primary structure of the Escherichia coli methionyl-tRNA synthetase gene

View Full Paper

Ask AI

Bookmark

To clone the metG gene from E. coli and analyze its product, methionyl-tRNA synthetase.
Cloned metG gene in plasmid pBR322 from an E. coli gene library.
Performed maxicell analysis to identify polypeptides expressed from pX1.
Conducted DNA sequencing for the metG gene and characterized its primary structure.
E. coli strain with plasmid pX1 produced methionyl-tRNA synthetase at a rate 40 times higher.
Identified a polypeptide of Mr 76,000 as methionyl-tRNA synthetase via immunological studies.
DNA sequencing revealed no significant repeated sequences and confirmed the metG region's homology between chromosome and episome.

No takes yet. Share an insight, caveat, or question.

Randomized trial shows methionyl-tRNA synthetase gene cloning in E. coli, indicating significant protein overproduction.

Dardel et al. (1984) studied this question.

View Full Paper

Ask AI

Bookmark