Key result
Mutations in the 307-311 region of the rat AT1a receptor, specifically phenylalanine309 and adjacent lysines, progressively decreased surface expression and inositol phosphate responses.
The highly conserved phenylalanine309 and adjacent lysine residues in the membrane-proximal region of the cytoplasmic tail are critical for the surface expression of the rat AT1a angiotensin receptor.
Answers are grounded in this paper and cite their sources.
Rat AT1a findings offer no clinical guidance; leaves open conservation of these residues in human receptors.
A series of deletion mutants was created to analyze the function of the membrane-proximal region of the cytoplasmic tail of the rat type 1a (AT1a) angiotensin receptor. In transiently transfected COS-7 cells, the truncated mutant receptors showed a progressive decrease in surface expression, with no major change in binding affinity for the peptide antagonist, [Sar1,Ile8]angiotensin II. In parallel with the decrease in receptor expression, a progressive decrease in angiotensin II-induced inositol phosphate responses was observed. Alanine substitutions in the region 307-311 identified the highly conserved phenylalanine309 and adjacent lysine residues as significant determinants of AT1a receptor expression.
No takes yet. Share an insight, caveat, or question.
Gáborik et al. (1998) studied this question. Deletion mutants and alanine substitutions in the membrane-proximal region of the cytoplasmic tail of the rat AT1a receptor was evaluated on Surface expression and angiotensin II-induced inositol phosphate responses. Mutations in the 307-311 region of the rat AT1a receptor, specifically phenylalanine309 and adjacent lysines, progressively decreased surface expression and inositol phosphate responses.
Synapse has enriched 10 related papers. Consider them for comparative context: