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Analyzing a drug in a completely overlapping spectrum is always a challenging and inevitable step in the analysis of multi-drug combinations. It requires sophisticated equipment that utilizes more hazardous solvents, which harms environmental safety. Adopting a simple, innovative, low-cost technique for determining compounds in a mixture without affecting the environment is necessary. This study aimed to resolve cilnidipine (CLD), chlorthalidone (CTD), and metoprolol (MTP) combination via an innovative spectrophotometric technique called successive ratio subtraction coupled with the constant multiplication (SRS-CM) and double divisor ratio first derivative method (DD-DD1) without any interference using propylene carbonate (PC) as a green biodegradable solvent. The determination of drugs has been performed in linear concentration ranges of 2.00–14.00, 2.50–11.25, and 2.50–32.50 µg/mL at λmax238.5, 228.5, and 223.2 nm for CLD, CTD, and MTP respectively for method I, and for CLD (243.46 nm), CTD (283.7 nm), and MTP (220.04 nm) using method II. Additionally, the method has been validated by the ICH Q2 R1 guidelines, evaluated through green analytical and sustainability indicators, and confirmed to meet rigorous standards for both environmental responsibility and analytical accuracy while aligning with relevant Sustainable Development Goals (SDGs).HighlightsMethod validated according to ICH Q2 R1 guidelines.Propylene carbonate, an eco-friendly solvent, was used.Assessed with green analytical tools and sustainability indicators, ensuring environmental responsibility.
Chanduluru et al. (Wed,) studied this question.